Monday 10 October 2011

PCR vs. DNA Sequencing (Sanger's Method)

Similarities:
- PCR and DNA Sequencing involve breaking the entire DNA Sequence into smaller fragments

Differences:
- DNA Sequencing requires special dideoxynucleotides (ddNTPs) to create fragments of the DNA of various lengths (lack of 3'-OH)
- DNA Sequencing uses Gel Electrophoresis to read the nucleotide sequence according to the various lengths
- The purpose of DNA Sequencing is to figure out the desired sequence
- The purpose of PCR is to replicate as many fragments as possible in the shortest amount of time

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