Monday 10 October 2011

PCR vs. Vector Cloning

Similarities:
- Both involve the isolation and replication of a specific gene, or short segment of DNA

Differences:
- PCR does not require the process of transformation
- Vector Cloning must utilize bacteria to replicate quickly, PCR does not require the use of any other cells
- PCR does not need expensive restriction enzymes, the desired gene is already isolated
- The purpose of Vector Cloning is to isolate a gene expression, PCR focuses on the speed and amount of replication

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